Decellularized biohybrid nerve promotes motor axon projections

Journal article

Abijeet Singh Mehta, Sophia L. Zhang, Xinran Xiea, Shreyaa Khanna, Joshua Tropp, Xudong Ji, Rachel Daso, Colin K. Franz, Sumanas W. Jordan, Jonathan Rivnay
Adv. Health. Mater., vol. 13(30), 2024, p. 2401875

DOI: 10.1002/adhm.202401875

Cite

APA Click to copy
Mehta, A. S., Zhang, S. L., Xiea, X., Khanna, S., Tropp, J., Ji, X., … Rivnay, J. (2024). Decellularized biohybrid nerve promotes motor axon projections. Adv. Health. Mater., 13(30), 2401875. https://doi.org/10.1002/adhm.202401875

Chicago/Turabian Click to copy
Mehta, Abijeet Singh, Sophia L. Zhang, Xinran Xiea, Shreyaa Khanna, Joshua Tropp, Xudong Ji, Rachel Daso, Colin K. Franz, Sumanas W. Jordan, and Jonathan Rivnay. “Decellularized Biohybrid Nerve Promotes Motor Axon Projections.” Adv. Health. Mater. 13, no. 30 (2024): 2401875.

MLA Click to copy
Mehta, Abijeet Singh, et al. “Decellularized Biohybrid Nerve Promotes Motor Axon Projections.” Adv. Health. Mater., vol. 13, no. 30, 2024, p. 2401875, doi:10.1002/adhm.202401875.

BibTeX Click to copy

@article{abijeet2024a,
  title = {Decellularized biohybrid nerve promotes motor axon projections},
  year = {2024},
  issue = {30},
  journal = {Adv. Health. Mater.},
  pages = {2401875},
  volume = {13},
  doi = {10.1002/adhm.202401875},
  author = {Mehta, Abijeet Singh and Zhang, Sophia L. and Xiea, Xinran and Khanna, Shreyaa and Tropp, Joshua and Ji, Xudong and Daso, Rachel and Franz, Colin K. and Jordan, Sumanas W. and Rivnay, Jonathan}
}

Developing nerve grafts with intact mesostructures, superior conductivity, minimal immunogenicity, and improved tissue integration is essential for the treatment and restoration of neurological dysfunctions. A key factor is promoting directed axon growth into the grafts. To achieve this, biohybrid nerves are developed using decellularized rat sciatic nerve modified by in situ polymerization of poly(3,4-ethylenedioxythiophene) (PEDOT). Nine biohybrid nerves are compared with varying polymerization conditions and cycles, selecting the best candidate through material characterization. These results show that a 1:1 ratio of FeCl3 oxidant to ethylenedioxythiophene (EDOT) monomer, cycled twice, provides superior conductivity (>0.2 mS cm−1), mechanical alignment, intact mesostructures, and high compatibility with cells and blood. To test the biohybrid nerve's effectiveness in promoting motor axon growth, human Spinal Cord Spheroids (hSCSs) derived from HUES 3 Hb9:GFP cells are used, with motor axons labeled with green fluorescent protein (GFP). Seeding hSCS onto one end of the conduit allows motor axon outgrowth into the biohybrid nerve. The construct effectively promotes directed motor axon growth, which improves significantly after seeding the grafts with Schwann cells. This study presents a promising approach for reconstructing axonal tracts in humans.